| عنوان فارسی مقاله: | سیلیمارین (silymarin) از طریق فسفرسیلاسیون ترئونین-286 سبب تخریب پروتئوزومی سیکلین D-1 در سلول های سرطانی کلورکتال می شود |
| عنوان انگلیسی مقاله: | Silymarin induces cyclin D1 proteasomal degradation via its phosphorylation of threonine-286 in human colorectal cancer cells |
چکیده
1.مقدمه
2.مواد و روش ها
1.2.مواد مورد استفاده
2.2 کشت سلولی و تیمار های سلولی
3.2 بررسی تکثیر و رشد سلولی
4.2. RT_PCR یا واکنش زنجیره ای پلیمراز- ترانس کریپتاز معکوس
5.2 SDS_PAGE و وسترن بلات
6.2 وکتور های بیانی
7.2. ترانسکفت موقتی و فعالیت لوسیفراز
8.2.آنالیز های آماری
3.نتایج
1.3.اثرات مهاری سیلامارین روی تکثیر و رشد سلول های HCT116 و SW480
2.3 اثرات مهاری سیلامارین بر بیان سیکلین D1 در سلول های HCT116 و SW480
3.3 تخریب پروتئوزومی سیکلین D1 با فسفریلاسیون ترئونین-286 به واسطه سیلیمارین
4.3.فعال سازی nk-kB منجر به تخریب پرتئوزومی سیکلین D1 به واسطه سیلامارین می شود.
4. بحث و گفت و گو
کلمات کلیدی :
Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its ... https://www.researchgate.net/.../14081283_Inhibition_of_cyclin_D1_phosphorylation_o... Official Full-Text Publication: Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway on ... Ubiquitination of Free Cyclin D1 Is Independent of Phosphorylation on ... www.jbc.org/content/275/16/12074.. by D Germain - 2000 - Cited by 103 - Related articles Cyclin D1 protein is ubiquitinated and degraded by the 26 S proteasome. ... cyclin D1 is ubiquitinated independently of its phosphorylation on threonine 286 by ... Ubiquitination of Free Cyclin D1 Is Independent of Phosphorylation on ... www.jbc.org/content/275/16/12074.full by D Germain - 2000 - Cited by 103 - Related articles Apr 21, 2000 - Phosphorylation of cyclin D1 on threonine 286 is required for its ubiquitination, ... Transfections were performed using Fugene6TM following the manufacturer's .... Cyclin D1 Is Degraded by the 26 S Proteasome in Vitro. [PDF]Silymarin induces cyclin D1 proteasomal degradation via its ... iranarze.ir/wp-content/uploads/2017/01/5922-English.pdf Silymarin induces cyclin D1 proteasomal degradation via its phosphorylation of threonine-286 in human colorectal cancer cells. Hyun Ji Eo a,1, Gwang Hun ... [PDF]Phosphorylation-Dependent Ubiquitination of Cyclin D1 by the SCF ... https://www.med.upenn.edu/.../Phosphorylation-dependentUbiquitinationofCyclinD1.... by DI Lin - 2006 - Cited by 283 - Related articles ... proteolysis. Degradation is triggered by threonine 286 phosphorylation, which promotes its ubiquitination by an unknown E3 ligase. ... ligase that promotes ubiquitin-dependent degradation ... necessity of Thr286 phosphorylation for cyclin D1 proteol- ysis (Diehl et ... D1, cells were treated with proteasome inhibitors to en-. The regulation of cyclin D1 degradation: roles in cancer development ... molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-6-24 by JP Alao - 2007 - Cited by 423 - Related articles Apr 2, 2007 - GSK3β phosphorylates threonine residue 286 (T286) and regulates cyclin D1 nuclear ... F. Free cyclin D1 is degraded through the ubiquitin dependent 26S ... cyclin D1 degradation via the 26S proteasome independently of ubiquitin [56]. G, H. GSK3β phosphorylates cyclin D1 on T286 which facilitates its ...